The recent identification of human embryonic stem cells offers an alternative source of cells for tissue engineering. However, researchers are still far from being able to control the differentiation of embryonic stem cells in culture. In addition, the research on embryonic cells brings up ethical problems. Therefore, a more immediate goal would be to isolate the so-called progenitor cells from tissues avoiding a potential immune response since they can be harvested from a small sample of the patient’s own marrow.

Under the activities of this research vector will address:

• The use genomics;
• The implementation of proteomics to improve the efficacy of use of stem cells and other progenitor cells;
• The development of adequate cell isolation, characterization and culturing methodologies. Specific cell culture techniques like e.g. Air-Liquid-Interface, perfusion and rotation culture;
• The development of culture human primary cells, differentiated cells or adult stem cell lines. A novel type of scaffold triggered by culture time and mechanical stimulation will accelerate cell proliferation and growth at the first culture stage and enhance the production of matrix protein at the later mechanical conditioning stage in vitro;
• The establishment of relevant in vitro models, such as three-dimensional cultures and co-cultures that would simulate the in vivo situation;
• The evaluation of the in vitro biocompatibility and performance of tissue engineering scaffolds focusing in the formation of functional tissue;
• The evaluating of effect of the growth medium on cell development with the intention of tuning the scaffold and medium to generate an environment that will produce functionally useful tissues.